Journal: bioRxiv
Article Title: Molecular and Structural Characterization Reveals Divergent Extracellular Vesicle Profiles Between Wild Type and Alzheimer’s Disease Cerebrocortical Organoids
doi: 10.64898/2026.05.13.724352
Figure Lengend Snippet: Organoid conditioned media was processed to isolate EVs yielding A and D) an EV-depleted Permeate, B and E) a High MW Protein sample, and C and F) a final EV isolate. The samples were adsorbed onto carbon formvar grids, negatively stained with uranyl acetate, and visualized by TEM. The scale bars in all images represent 100 nm. Morphological analysis of WT and AD organoid derived EVs was also carried out with cryo-TEM . G) Micrographs of wildtype EVs. H ) Vesicular area distribution for 24 WT EVs and 65 AD EVs. I) Micrographs of AD EVs. Scale bar 50 nm, multilamellar vesicles indicated with blue arrows, fibrils or amyloids indicated with red arrows.
Article Snippet: Excess sample was wicked away and grids were negatively stained with 2% uranyl= acetate (Ted Pella, Inc, 19481)□for 2 minutes.
Techniques: Staining, Derivative Assay